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Preparation of orientational silk fibroin hydrogels(PDF)

Journal of Silk [ISSN:1001-7003] [CN:33-1122/TS]

Research Field:
Research and Technolgy
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Preparation of orientational silk fibroin hydrogels
CHEN Daqi FU Hua YIN Zhuping WU Feng XUE Xiang LU Shenzhou
 a.College of Textile and Clothing Engineering; b.National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China
silk fibroin bacillus subtilis sodium surfactin shear force orientation hydrogels mechanical property
In this study, silk fibroin (SF) was adopted as raw material , bacillus subtilis sodium surfactin (SS) was blended with silk fibroin to shorten the g elation time of SF solution, and mechanical shear force was exerted on the blended solution of SF/SS in the gelation process to prepare an orientational SF/SS hydrogel. The results suggest that the molecular conformation of the SF/SS blending system takes on random coil it is subject to shearing for 0min to 20min at the rate of 55 s-1, while the molecular conformation transforms from random coil into β-sheet conformers during the period that it is subject to shearing for 20min until completely gelling; the shear-induced SF/SS hydrogels appears to be of obvious orientational gel skeleton/network morphology, its compressive strength in the direction of orientation is 3.5 times over that of unsheared hydrogel in the direction of non-orientation, and its cut resistance in the direction vertical to the direction of orientation is twice over that of unsheared hydrogel in the direction of orientation. SF/SS orientational hydrogels can be applied for culture of nerve cell and bone cell, and repairing of defect in muscle and ligament tissues.


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Last Update: 2017-06-30