|本期目录/Table of Contents|

[1]寿鑫,应慧慧,李擎,等.家蚕杆状病毒表达系统研究进展[J].丝绸,2012,49(09):000018-22.
 SHOU Xin,YING Hui-hui,LI Qing,et al.Progress of researches on baculovirus expression system of bombyx mori[J].Journal of Silk,2012,49(09):000018-22.
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家蚕杆状病毒表达系统研究进展(PDF)
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《丝绸》[ISSN:1001-7003/CN:33-1122/TS]

卷:
49
期数:
2012年09期
页码:
000018-22
栏目:
研究与技术
出版日期:
2012-09-20

文章信息/Info

Title:
Progress of researches on baculovirus expression system of bombyx mori
文章编号:
1001-7003(2012)09-0018-05
作者:
寿鑫 1应慧慧 1李擎 1于威 12张耀洲 12
1.浙江理工大学 生命科学学院,杭州 310018;2.浙江省家蚕生物反应器和生物医药重点实验室,杭州 310018
Author(s):
SHOU Xin 1 YING Hui-hui 1 LI Qing 1 YU Wei 1 2 ZHAGN Yao-zhou 1 2
1.College of Life Science, Zhejiang Sci-Tech University, Hangzhou 310018, China; 2.Zhejiang Provincial Key Laboratory of Silkworm Bioreactor and Biomedicine, Hangzhou 310018, China
关键词:
家蚕杆状病毒表达系统新进展
Keywords:
Bombyx mori Baculovirus expression system New progress
分类号:
S881.24
doi:
-
文献标志码:
A
摘要:
近年来家蚕杆状病毒表达系统在应用和优化等方面研究有了新进展。家蚕杆状病毒表达系统利用携带外源目的蛋白基因的重组杆状病毒对家蚕及其细胞系的高效感染能力,在感染后的家蚕体内或培养细胞中大量表达目的蛋白。家蚕作为
一种外源蛋白表达载体,具有与哺乳动物细胞类似的翻译后修饰过程,其与核型多角体病毒的动态相互作用也一直是研究的热点。由于该系统潜在的巨大优势,为生产人类急需的蛋白质药物、基因工程疫苗和基因工程杀虫剂等提供了新的途径。
Abstract:
Based on application and optimization of baculovirus expression system of bombyx mori, this thesis summarizes the progress of latest researches on baculovirus expression system of bombyx mori. The expression system of bombyx mori baculovirus uses the high-efficiency infectivity of recombinant rhabdovirus carrying exogenous objective protein gene production on its cell lines to express a large number of objective proteins in infected bombyx mori or cultivated cells. As an expression vector of exogenous protein, bombyx mori has post-translational modifications like the mammalian cells and the dynamic interaction with Bombyx mori nucleopolyhedrovirus has been a hot research field all the time. As the system has potential huge advantages, it provides new approaches for protein medicine, genetic engineering vaccine and genetic engineering insecticide which people need urgently.

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备注/Memo

备注/Memo:
收稿日期:2012-06-07
基金项目:国家高技术研究发展计划“863”项目(2011AA100603);浙江省自然科学基金项目(Y3090339);浙江省大学生科技创新计划项目(2011R406028)
作者简介:寿鑫(1990- ),男,2009级生物技术专业本科生。
通讯作者:于威,副教授,硕导,yuwei@zstu.edu.cn
更新日期/Last Update: 2012-10-10